A small fleet of fishermen operates from the Harbour, often fishing in the wider Solent.  Fishing activities are regulated by the Southern Inshore Fisheries and Conservation Authority (SIFCA).

The Harbour is a nursery for bass and the health of the juvenile fish is monitiored and recorded regularly by SIFCA.  There is a small oyster fishery which is also monitored by the Chichester Harbour Oyster Partnership Initiative (CHOPI)-  a partnership comprising the Conservancy, Sussex IFCA and Natural England.